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INULINASE PURIFICATION PDF

Keywords: Characterization; Inulinase; Production; Purification; Streptomyces. 1. Introduction. Inulin is a polyfructan in plant consisting of linear chains. Aspergillus niger exo-inulinase purification by three phase partitioning. Inulinase (2, 1-β-D-fructan fructanohydrolase, EC ) hydrolyses inulin into. 42(4), August , pp. Purification and characterization of Inulinase from marine bacterium,. Bacillus cereus MU S Meenakshi1, S Umayaparvathi .

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Therefore, this study aimed to collate all information, particularly on fungal sources, characteristics, and applications of inulinases. Effect of different substrate concentration on the purified inulinase produced by Streptomyces grisenus. It was identified and confirmed according to description of ISP reported by Shirling and Gottliebaband and key of Bergey’s Manual of Williams et al.

At the end of incubation period, the cells were separated by centrifugation at rpm for 20 min under cooling and the clear supernatant crude extract was used for enzyme purification. After separation, the proteins were transferred to a polyvinylidene difluoride membrane, and after staining with ponceau S solution, the inulinase protein band was cut from the membrane and subjected to amino acid sequence analysis. GNDuI produced high levels of extra cellular inlinase after 24 h at pH 7. Pakistan Journal of Biological Sciences Volume 9 5: As shown in Table 1 the maximum activity was found on the 2nd day of incubation period.

Co-operative description of type culture of Streptomyces sp. Purification and Characterization of Exoinulinase Enzyme from Streptomyces grisenus. Isolation of mutant of Kluyeromyces sp.

The eluate was assayed for protein content and inulinase activity.

Furthermore, this review highlights the various physico-chemical characteristics of fungal inulinases to determine their suitability for industrial use. Idenlification of prokaryotic and eukaryotic signal peptides and predication of their cleavage site. The effect of carbon source on inulinase production was shown in Table 4.

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Similar results were obtained YoKota et al. Oligosaccharides ibulinase compounds with great potential of use in food industry particularly interesting are fructooligosaccharids FOSbecause of their favorable functional properties such as low calorie and noncariogenic sweeteners, improvement of the intestinal microbial flora, relief of constipation, decrease of total cholesterol and lipid in the serum and promotion of animal growth Vandamme and Derycke, Elution was carried out by 0.

Optimisation of inulinase production by Kluyveromyces bulgaricus. In uprification, extended data on inulinase producing fungal strains have also been reviewed.

Production, Purification, Characterization and Applications of Fungal Inulinases | BenthamScience

These results are similar to those obtained by yokota et al. In this substantial review, each screened paper is described and its findings are proposed as viewpoints. Effect of different pH values on the inulinase activity of Streptomyces grisenus. Besides, various promising applications and aspects of enzymatic hydrolysis for producing important industrial products also have been discussed.

Y resistant to catabolite repression. Inulin can inluinase considered as dietary fibre, a substitute for fat and low calorie sweetener Roberfroid, Representing the results of fraction 50 fraction of inuline lyase enzyme using the chromatograph resulted in one sharp peak and the molecular weight was obtained KDa.

In order to determine the N-terminal amino acid sequence, the protein was extracted from streptomyces grisenus cells by 8 M urea treatment and purificztion separated in an SDS-PAGE gel as already described Laemmli, Purification, ijulinase and comparison of invertase, exoinulinase and endoinulinase of Aspergillus ficuum.

The purification steps of extracellular inulinase from Streptomyces grisenus. Inulinases are important industrial hydrolysing enzymes which are generally used for the production of high fructose syrup and fructooligosaccharides. These results agreement with Abeer indicated that the activity of inulinase by streptomyces griseus increased with increasing the sub-state concentration until 1 mu.

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The Biology of ActinomycetesMordarski, M. Problems of Classification of Actinomycetes Antagonists. Volume 7Issue 3 Purification and characterizationof an endoinulinase from Xanthomonas oryzae No.

The results in Table 2 revealed that the maximum activity was observed at pH 7. Thermal stability of inulinase immobilized on DEAE cellulose with or without glulardehyde. Present findings are in agreement with Lim et al. Cite this article as: Elution profile of the enzyme produced streptomyces grisenus after sephadex G filtration. Hydrolysis of inulin from jerusalem artichake by inulinase immobilized on aminoethyl cellulose enzyme.

Effect of incubation period purificatipn the inulinase activity of Streptomyces grisenus. Pakistan Journal of Biological Sciences, 9: Co-operative description of type culture of St.

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Methods for characterization of Streptomyces species. Protein measurement with the folin phenol reagent. The medium used for the production of inulinase contained 8 L -1 according to Lim et al.

Therefore, microbial inulinases which can be induced by growing microorganisms, have a potential for industrial use in the production of fructose frminulin Edelman and Jefford, Its production, properties and industrial applications. Gel filtration using sephadex G Expression, purification and characterization of an exo-B-D-Fructosidase purificatikn Streptomyces mutans. The production broth medium was optimized for maximum inulinase production by addition of 0. The results in Table 6 and 7 revealed the effect of some physical conditions on purified enzyme produced by purificaton grisenus viz.

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